Coding

Part:BBa_K2608006:Design

Designed by: Guijie Ren   Group: iGEM18_JNFLS   (2018-10-07)


truncated and optimized HCV core protein (173 aa) with part of cspA 5’UTR sequence and TEE, 6xHis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 273
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 490
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

For expression efficiently,it was truncated as 173aa and codons were optimized.

Source

HCV core protein cDNA.

References